黄体酮对雌性家兔十二指肠肌电活动作用的数学模型研究

doi:10.11843/j.issn.0366-6964.2018.12.021

摘要/Abstract

摘要：

为研究孕激素对十二指肠平滑肌活动的作用特点，并构建相应的数学模型，以揭示其作用机制，笔者以黄体酮给药剂量0、0.5、1.0、1.5和2.0 mg·kg^-1进行试验分组，给予卵巢摘除和电极埋置的家兔动物模型相应剂量的黄体酮肌内注射处理，采用BL-420F生物机能试验系统采集试验组家兔十二指肠的肌电活动变化，并以肌电活动指数作为因变量，黄体酮剂量为自变量构建数学模型，来揭示孕激素对十二指肠平滑肌活动的作用机制。结果表明：1）黄体酮剂量在剂量范围为1.0~2.0 mg·kg^-1内，十二指肠肌电的振幅、频率与肌电活动指数均随黄体酮剂量的增加呈现出一种先升高后降低的变化趋势，即浓度依赖性调节模式；2）构建黄体酮剂量和肌电活动指数的函数模型，符合高斯函数的基本变化特征，函数拟合精度分析结果显示该模型的R²=0.998 0，P=0.001 4，表明模型拟合精度极高；3）由该函数可知在黄体酮浓度范围为0.72~1.98 mg·kg^-1内，肌电活动指数发生显著变化，其变化范围为1.64~4.92 mV·min^-1。同时以黄体酮剂量分别为1.14、1.35、1.56 mg·kg^-1为分界点，肌电活动指数的变化趋势表现为连续的指数型增加、对数型增加、指数型减小以及对数型减小。上述结果说明，黄体酮对十二指肠平滑肌电的活动的调节作用是在十二指肠本身具备一定水平的肌电活动的基础上进行的，即黄体酮是十二指肠肌电活动的间接调节者。本试验的研究结果为阐明孕激素调节十二指肠运动的作用方式以及孕激素类药物的研发和使用均提供了十分重要的数据化理论依据和参考。

Abstract:

This study was conducted to explore the characteristics of progesterone on duodenal smooth muscle activity and to establish a mathematical model to reveal its mechanism. In this experiment, rabbits were grouped according to the dosage of progesterone containing 0, 0.5, 1.0, 1.5 and 2.0 mg·kg^-1. The corresponding dose of progesterone was injected intramuscularly into the ovariectomized and electrode implanted rabbit models. The changes of myoelectric activity in the duodenum of the experimental group were collected by BL-420F biological function test system. Using myoelectric activity index as dependent variable and progesterone dose as independent variable, a mathematical model was constructed to reveal the mechanism of progesterone on duodenal smooth muscle activity. The results showed that:1) In the dose range of 1.0-2.0 mg·kg^-1, the amplitude, frequency and myoelectric activity index of duodenal myoelectric activity increased firstly and then decreased with the increase of progesterone dose, that is, concentration-dependent regulation mode. 2) A functional model of progesterone dosage and myoelectric activity index was constructed, which accord with the basic change characteristics of Gauss function. The accuracy of the function fitting analysis showed that the R²=0.998 0 and P=0.001 4, suggesting the fitting accuracy of the model was very high. 3) According to this function, the myoelectric activity index changed significantly in the range of 0.72-1.98 mg kg^-1 of progesterone, ranging from 1.64 to 4.92 mV min^-1. At the same time, the dosage of progesterone of 1.14, 1.35 and 1.56 mg·kg^-1 were the boundary point. The change trend of myoelectric activity index was continuous exponential type increase, logarithmic type increase, exponential type decrease and logarithmic type decrease. The above results suggest that the regulation of progesterone on the electrical activity of duodenal smooth muscle is based on a certain level of myoelectric activity in the duodenum. The results of this study provide a very important theoretical basis and reference for clarifying the mode of progesterone regulating duodenal movement and the development and use of progesterone drugs.

中图分类号:

S852.2

杜宜楠, 辛先萌, 赵建帅, 张金, 黄双, 郭雅茹, 张莹利, 徐永平. 黄体酮对雌性家兔十二指肠肌电活动作用的数学模型研究[J]. 畜牧兽医学报, 2018, 49(12): 2715-2722.

DU Yi-nan, XIN Xian-meng, ZHAO Jian-shuai, ZHANG Jin, HUANG Shuang, GUO Ya-ru, ZHANG Ying-li, XU Yong-ping. The Study of Mathematic Model of Progesterone on the Regulation of Duodenal Myoelectric Activity of Female Rabbit[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2715-2722.

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